Stages of oocyte development in G. rufa (a) Oogonium x1500 PAS (periodic acid-Schiff stain).(b) Chromatin nucleolus stage x1500 PAS. (c) Peri-nucleolar stage x600 H&E (Harris' hematoxylin eosin) (d) Cortical alveolar stage x300 PAS. (e) Vitellogenic stage x150 PAS (f) Vitellin membrane x1500 H&E. (g) Maturation stage x150PAS. (h) Atretic oocyte x600 H&E.
Abbreviations: O, Oogonium; PN, Primer Nucleolus; CR, Chromatin; N, Nucleus; YN, Yolk nucleus; CA, Cortical alveoles; GV, Germinal vessicle; YV, Yolk vessicles; TL, Teca layer; GL, Granulosa layer; ZR, Zona radiata; MO, Mature oocyte