FIGURE 2
Phase contrast images of growing hyphal tip of R. solani photographed
at 30 sec intervals to show the typical apical organization. The Spitzenkörper
(s) is composed of two distinct regions, an inner bright core and an outer
dark zone. Note the apparent changes in shape of the bright core from frame
to frame. Lipid bodies or spherosomes (sp), the very dark round objects,
are prominent in the subapical cytoplasm. Mitochondria (m), the medium dark
filamentous structures, are found in a compact cluster immediately behind
the Spitzenkörper and throughout the subapical cytoplasm. Mitochondria
are typically oriented parallel to the long axis of the growing hypha. Small
bright components are also scattered in the cytoplasm and on occasion seem
to coalesce with each other or with the subapical periphery of the Spitzenkörper
core (60 sec view). Animation. Scale bar = 10 µm.