Native-PAGE of fractions of RBPs after preparative
isoelectric focusing in a Rotofor cell in 1.1% ampholyte (pH 4.5-5.4).
The end conditions after a 4 h fractionation were 1400 V and 8
mA at 12 W constant power. Lanes 1 and 8 contained RBPs prior
to Rotofor fractionation. Lanes 2-7 contained Rotofor compartment
fractions 14-9, respectively).