FIGURE 1

A computer model of our original prediction of the GR recognition helix (2) (right) compared to a computer model from NMR derived coordinates of the GR recognition helix (3) (left). End views of the alpha helices are shown with amino acids designated using the Dayhoff (8) one letter code and numbered as in the rat GR (1). Main chains are colored grey, color coding of side chains is based on polarity: positively charged sidechains = blue, negatively charged side chains = red, uncharged polar side chains = yellow and nonpolar side chains = purple. The Root Mean Square (RMS) deviations for the two structures are 1.25 angstroms for the alpha carbon atoms (11 atoms), 1.57 angstroms for the main chain atoms (44 atoms), and 2.31 angstroms overall (76 atoms).